Single-cell gene regulatory network analysis reveals potential mechanisms of action of antimalarials against SARS-CoV-2 Institutional Repository Document uri icon


  • The efficiency of antimalarials, chloroquine (CQ) and hydroxychloroquine (HCQ), in the prevention and treatment of coronavirus disease 2019 (COVID-19) is under intense debate. The mechanisms of action of antimalarials against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have not been fully elucidated. Here, we applied a network-based comparative analysis, implemented in our machine learning workflowscTenifoldNet, to scRNA-seq data from COVID-19 patients with different levels of severity. We found that genes of the Malaria pathway expressed in macrophages are significantly differentially regulated between patients with moderate and severe symptoms. Our findings help reveal the mechanisms of action of CQ and HCQ during SARS-CoV-2 infection, providing new evidence to support the use of these antimalarial drugs in the treatment of COVID-19, especially for patients who are mildly affected or in the early stage of the infection.

altmetric score

  • 2.75

author list (cited authors)

  • Cai, J. J., & Hurtado, D.

citation count

  • 1

complete list of authors

  • Cai, James J||Hurtado, Daniel Camilo Osorio

Book Title

  • OSF Preprints

publication date

  • July 2020