iPSCs and fibroblast subclones from the same fibroblast population contain comparable levels of sequence variations Academic Article uri icon

abstract

  • Significance One important unsolved question in the stem cell field is, do induced pluripotent stem cells (iPSCs) have more mutations than other cultured somatic cells because of the reprogramming process? In this work, we took a novel approach to interrogate the genome integrity of iPSCs by comparing mutational load of clonal fibroblast lines and iPSC lines derived from the same fibroblast parental cells. Whole exome sequencing demonstrates that iPSCs and clonal fibroblasts have comparable numbers of new mutations, as compared with their parental fibroblasts. Deep, targeted resequencing also shows that greater than 90% of these mutations are random, preexisting sequence variants in small subsets of the parental fibroblast population. Our data strongly suggest that reprogramming process is not mutagenic.

published proceedings

  • Proceedings of the National Academy of Sciences

altmetric score

  • 63.25

author list (cited authors)

  • Kwon, E. M., Connelly, J. P., Hansen, N. F., Donovan, F. X., Winkler, T., Davis, B. W., ... Liu, P.

citation count

  • 46

complete list of authors

  • Kwon, Erika M||Connelly, John P||Hansen, Nancy F||Donovan, Frank X||Winkler, Thomas||Davis, Brian W||Alkadi, Halah||Chandrasekharappa, Settara C||Dunbar, Cynthia E||Mullikin, James C||Liu, Paul

publication date

  • February 2017