Dog10K_Boxer_Tasha_1.0: A Long-Read Assembly of the Dog Reference Genome Academic Article uri icon

abstract

  • The domestic dog has evolved to be an important biomedical model for studies regarding the genetic basis of disease, morphology and behavior. Genetic studies in the dog have relied on a draft reference genome of a purebred female boxer dog named “Tasha” initially published in 2005. Derived from a Sanger whole genome shotgun sequencing approach coupled with limited clone-based sequencing, the initial assembly and subsequent updates have served as the predominant resource for canine genetics for 15 years. While the initial assembly produced a good-quality draft, as with all assemblies produced at the time, it contained gaps, assembly errors and missing sequences, particularly in GC-rich regions, which are found at many promoters and in the first exons of protein-coding genes. Here, we present Dog10K_Boxer_Tasha_1.0, an improved chromosome-level highly contiguous genome assembly of Tasha created with long-read technologies that increases sequence contiguity >100-fold, closes >23,000 gaps of the CanFam3.1 reference assembly and improves gene annotation by identifying >1200 new protein-coding transcripts. The assembly and annotation are available at NCBI under the accession GCF_000002285.5.

published proceedings

  • Genes

altmetric score

  • 14.7

author list (cited authors)

  • Jagannathan, V., Hitte, C., Kidd, J. M., Masterson, P., Murphy, T. D., Emery, S., ... Wang, G.

citation count

  • 1

complete list of authors

  • Jagannathan, Vidhya||Hitte, Christophe||Kidd, Jeffrey M||Masterson, Patrick||Murphy, Terence D||Emery, Sarah||Davis, Brian||Buckley, Reuben M||Liu, Yan-Hu||Zhang, Xiang-Quan||Leeb, Tosso||Zhang, Ya-Ping||Ostrander, Elaine A||Wang, Guo-Dong

publication date

  • May 2021

published in