Alveolar Bone Marrow Gli1+ Stem Cells Support Implant Osseointegration. Academic Article uri icon


  • Osseointegration is the key issue for implant success. The in vivo properties of cell populations driving the osseointegration process have remained largely unknown. In the current study, using tissue clearing-based 3-dimensional imaging and transgenic mouse model-based lineage tracing methods, we identified Gli1+ cells within alveolar bone marrow and their progeny as the cell population participating in extraction socket healing and implant osseointegration. These Gli1+ cells are surrounding blood vessels and do not express lineage differentiation markers. After tooth extraction and delayed placement of a dental implant, Gli1+ cells were activated into proliferation, and their descendants contributed significantly to new bone formation. Ablation of Gli1+ cells severely compromised the healing and osseointegration processes. Blockage of canonical Wnt signaling resulted in impaired recruitment of Gli1+ cells and compromised bone healing surrounding implants. Collectively, these findings demonstrate that Gli1+ cells surrounding alveolar bone marrow vasculature are stem cells supporting dental implant osseointegration. Canonical Wnt signal plays critical roles in regulating Gli1+ stem cells.

published proceedings

  • J Dent Res

author list (cited authors)

  • Yi, Y., Stenberg, W., Luo, W., Feng, J. Q., & Zhao, H.

citation count

  • 7

complete list of authors

  • Yi, Y||Stenberg, W||Luo, W||Feng, JQ||Zhao, H

publication date

  • January 2022