Analytical glycobiology at high sensitivity: current approaches and directions. Academic Article

abstract

• This review summarizes the analytical advances made during the last several years in the structural and quantitative determinations of glycoproteins in complex biological mixtures. The main analytical techniques used in the fields of glycomics and glycoproteomics involve different modes of mass spectrometry and their combinations with capillary separation methods such as microcolumn liquid chromatography and capillary electrophoresis. The need for high-sensitivity measurements have been emphasized in the oligosaccharide profiling used in the field of biomarker discovery through MALDI mass spectrometry. High-sensitivity profiling of both glycans and glycopeptides from biological fluids and tissue extracts has been aided significantly through lectin preconcentration and the uses of affinity chromatography.

authors

• Alley, William

published proceedings

• Glycoconj J

author list (cited authors)

• Novotny, M. V., Alley, W. R., & Mann, B. F.

citation count

• 55

complete list of authors

• Novotny, Milos V||Alley, William R||Mann, Benjamin F

publication date

• January 2013

publisher

• Springer Nature  Publisher

published in

• Glycoconjugate Journal  Journal

keywords

• Chromatography, Affinity
• Glycomics
• Glycoproteins
• Humans
• Limit Of Detection
• Mass Spectrometry

PubMed Central ID

• 22945852

Digital Object Identifier (DOI)

• 10.1007/s10719-012-9444-8

start page

• 89

end page

• 117

volume

• 30

issue

• 2

URL

• http://dx.doi.org/10.1007/s10719-012-9444-8