SPP1 expression in the mouse uterus and placenta: implications for implantation. Academic Article uri icon


  • Secreted phosphoprotein 1 (SPP1, also known as osteopontin) binds integrins to mediate cell-cell and cell-extracellular matrix communication to promote cell adhesion, migration, and differentiation. Considerable evidence links SPP1 to pregnancy in several species. Current evidence suggests that SPP1 is involved in implantation and placentation in mice, but in vivo localization of SPP1 and in vivo mechanistic studies to substantiate these roles are incomplete and contradictory. We localized Spp1 mRNA and protein in the endometrium and placenta of mice throughout gestation, and utilized delayed implantation of mouse blastocysts to link SPP1 expression to the implantation chamber. Spp1 mRNA and protein localized to the endometrial luminal (LE), but not glandular epithelia (GE) in interimplantation regions of the uterus throughout gestation. Spp1 mRNA and protein also localized to uterine naturel killer (uNK) cells of the decidua. Within the implantation chamber, Spp1 mRNA localized only to intermittent LE cells, and to the inner cell mass. SPP1 protein localized to intermittent trophoblast cells, and to the parietal endoderm. These results suggest that SPP1: (1) is secreted by the LE at interimplantation sites for closure of the uterine lumen to form the implantation chamber; (2) is secreted by LE adjacent to the attaching trophoblast cells for attachment and invasion of the blastocyst; and (3) is not a component of histotroph secreted from the GE, but is secreted from uNK cells in the decidua to increase angiogenesis within the decidua to augment hemotrophic support of embryonic/fetal development of the conceptus.

published proceedings

  • Biol Reprod

altmetric score

  • 0.5

author list (cited authors)

  • Kramer, A. C., Erikson, D. W., McLendon, B. A., Seo, H., Hayashi, K., Spencer, T. E., ... Johnson, G. A.

citation count

  • 1

complete list of authors

  • Kramer, Avery C||Erikson, David W||McLendon, Bryan A||Seo, Heewon||Hayashi, Kanako||Spencer, Thomas E||Bazer, Fuller W||Burghardt, Robert C||Johnson, Greg A

publication date

  • October 2021