Menaquinone Biosynthesis: New Strategies to Trap Radical Intermediates in the MqnE-Catalyzed Reaction.
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Aminofutalosine synthase (MqnE) is a radical SAM enzyme that catalyzes the conversion of 3-((1-carboxyvinyl)oxy)benzoic acid to aminofutalosine during the futalosine-dependent menaquinone biosynthesis. In this Communication, we report the trapping of a radical intermediate in the MqnE-catalyzed reaction using sodium dithionite, molecular oxygen, or 5,5-dimethyl-1-pyrroline-N-oxide. These radical trapping strategies are potentially of general utility in the study of other radical SAM enzymes.
author list (cited authors)
Joshi, S., Fedoseyenko, D., Sharma, V., Nesbit, M. A., Britt, R. D., & Begley, T. P.
complete list of authors
Joshi, Sumedh||Fedoseyenko, Dmytro||Sharma, Vishav||Nesbit, Mark A||Britt, R David||Begley, Tadhg P