Menaquinone Biosynthesis: New Strategies to Trap Radical Intermediates in the MqnE-Catalyzed Reaction.

abstract

• Aminofutalosine synthase (MqnE) is a radical SAM enzyme that catalyzes the conversion of 3-((1-carboxyvinyl)oxy)benzoic acid to aminofutalosine during the futalosine-dependent menaquinone biosynthesis. In this Communication, we report the trapping of a radical intermediate in the MqnE-catalyzed reaction using sodium dithionite, molecular oxygen, or 5,5-dimethyl-1-pyrroline-N-oxide. These radical trapping strategies are potentially of general utility in the study of other radical SAM enzymes.

authors

• Begley, Tadhg

published proceedings

• Biochemistry

altmetric score

• 6.45

author list (cited authors)

• Joshi, S., Fedoseyenko, D., Sharma, V., Nesbit, M. A., Britt, R. D., & Begley, T. P.

citation count

• 3

complete list of authors

• Joshi, Sumedh||Fedoseyenko, Dmytro||Sharma, Vishav||Nesbit, Mark A||Britt, R David||Begley, Tadhg P

publication date

• June 2021

publisher

• American Chemical Society (ACS)  Publisher

published in

• Biochemistry  Journal

keywords

• Catalysis
• Electron Spin Resonance Spectroscopy
• Free Radicals
• Nucleosides
• Oxygen
• Vitamin K 2

PubMed Central ID

• 33999605

Digital Object Identifier (DOI)

• 10.1021/acs.biochem.1c00181

start page

• 1642

end page

• 1646

volume

• 60

issue

• 21

URL

• http://dx.doi.org/10.1021/acs.biochem.1c00181