Assessment of Plasma Membrane Fatty Acid Composition and Fluidity Using Imaging Flow Cytometry. Academic Article

abstract

• Phospholipid fatty acid (FA) composition influences the biophysical properties of the plasma membrane and plays an important role in cellular signaling. Our previous work has demonstrated that plasma membrane fatty acid composition is an important determinant of oncogenic Ras signaling and that dietary (exogenous) modulation of membrane composition may underlie the chemoprotective benefits of long chain n-3 polyunsaturated fatty acids (PUFA). In this chapter, we describe in vitro methods to modulate membrane phospholipid fatty acid composition of cultured cells using fatty acids complexed to bovine serum albumin (BSA). Furthermore, we describe a method to quantify the biophysical properties of plasma membranes in live cells using Di-4-ANEPPDHQ (Di4) and image-based flow cytometry.

authors

• Chapkin, Robert

published proceedings

• Methods Mol Biol

author list (cited authors)

• Fuentes, N. R., Salinas, M. L., Wang, X., Fan, Y., & Chapkin, R. S.

citation count

• 2

publication date

• January 2021

publisher

• Springer Nature  Publisher

keywords

• Animals
• Cattle
• Cell Membrane
• DHA
• Di-4-annepdhq
• Fatty Acids
• Flow Cytometry
• Imaging Flow Cytometry
• Lipid Rafts
• Membrane Fluidity
• Membrane Order
• N-3 PUFA
• Phospholipids
• Serum Albumin, Bovine

Digital Object Identifier (DOI)

• 10.1007/978-1-0716-1190-6_14

start page

• 251

end page

• 258

volume

• 2262

URL

• http://dx.doi.org/10.1007/978-1-0716-1190-6_14