Synergistic Antitumor Activity of Gramicidin/Lipophilic Bismuth Nanoparticles (BisBAL NPs) on Human Cervical Tumor Cells Academic Article uri icon


  • The objective of this study was to study the synergistic antitumor effect of lipophilic bismuth nanoparticles (BisBAL NPs) with the antibiotic solution Neo-Poly gramicidin on human cervical tumor cells. The effect of BisBAL NPs and Neo-Poly gramicidin solution on cervical cancer cell line (HeLa) was determined by the MTT cell viability assay and fluorescence microscopy. After a 24-h exposure to 0.1 Neo-Poly gramicidin HeLa cell growth decreased 94%. Fluorescence microscopy confirmed the antitumor effect cell death was higher among treated than among non-treated cells cells. Individually, gramicidin (0.04 mg/mL) inhibited HeLa tumor cell growth most (40%), and neomycin (0.04 mg/mL) least (21%). Gramicidin (0.3 mg/mL) in combination with different concentrations (1150 M) of BisBAL NPs had a synergistic antitumor effect against HeLa cells, reaching an > 86% tumor growth inhibition. As far as we know, we are the first to describe the antitumor activity of the antibiotic Neo-Poly gramicidin on a human cervical cancer cell line. The action mechanism of gramicidin/BisBAL NP is based on a strong damage on cell membrane and nucleus of tumor cells. A synergistic effect of gramicidin with BisBAL NPs may be useful as an alternative therapy for cervical cancer patients.

published proceedings

  • Frontiers in Nanotechnology

altmetric score

  • 1

author list (cited authors)

  • Cabral-Romero, C., Garca-Cuellar, C. M., Hernandez-Delgadillo, R., Snchez-Prez, Y., Meester, I., Sols-Soto, J. M., Pineda-Aguilar, N., & Chellam, S.

citation count

  • 0

complete list of authors

  • Cabral-Romero, Claudio||García-Cuellar, Claudia María||Hernandez-Delgadillo, Rene||Sánchez-Pérez, Yesennia||Meester, Irene||Solís-Soto, Juan Manuel||Pineda-Aguilar, Nayely||Chellam, Shankararaman

publication date

  • April 2021