Guanine-rich telomeric sequences stimulate DNA polymerase activity in vitro. Academic Article

abstract

• Guanine-rich oligonucleotides and short telomeric DNA sequences can self-associate into G-quartet stabilized complexes. We discovered that this self-association can occur in sequencing reactions and that higher-order structures stimulate DNA polymerase to synthesize extended DNA strands. Base analogues were used to identify Hoogsteen base pairings as stabilizing forces in these stimulatory DNA structures. Scanning force microscopy confirmed that quartet-DNA was formed from these oligomers and that these extended, four-stranded structures could be bound by DNA polymerase. Since guanine quartet-stabilized structures are proposed to exist in vivo, such structures may stimulate DNA polymerization in vivo.

authors

• Bradley, Robert Kelley

published proceedings

• Biochemistry

author list (cited authors)

• Ying, J., Bradley, R. K., Jones, L. B., Reddy, M. S., Colbert, D. T., Smalley, R. E., & Hardin, S. H.

citation count

• 8

publication date

• January 1999

publisher

• American Chemical Society (ACS)  Publisher

published in

• Biochemistry  Journal

keywords

• 2-aminopurine
• Animals
• Base Pairing
• DNA
• DNA Replication
• DNA-Directed DNA Polymerase
• Deoxyguanine Nucleotides
• Electrophoresis, Polyacrylamide Gel
• Guanine
• Humans
• Hypotrichida
• Oligonucleotides
• Saccharomyces Cerevisiae
• Sequence Analysis, DNA
• Telomere
• Tetrahymena

PubMed Central ID

• 10600107

Digital Object Identifier (DOI)

• 10.1021/bi9918543

start page

• 16461

end page

• 16468

volume

• 38

issue

• 50

URL

• http://dx.doi.org/10.1021/bi9918543