Sequencing of allotetraploid cotton (Gossypium hirsutum L. acc. TM-1) provides a resource for fiber improvement Academic Article uri icon

abstract

  • Upland cotton is a model for polyploid crop domestication and transgenic improvement. Here we sequenced the allotetraploid Gossypium hirsutum L. acc. TM-1 genome by integrating whole-genome shotgun reads, bacterial artificial chromosome (BAC)-end sequences and genotype-by-sequencing genetic maps. We assembled and annotated 32,032 A-subgenome genes and 34,402 D-subgenome genes. Structural rearrangements, gene loss, disrupted genes and sequence divergence were more common in the A subgenome than in the D subgenome, suggesting asymmetric evolution. However, no genome-wide expression dominance was found between the subgenomes. Genomic signatures of selection and domestication are associated with positively selected genes (PSGs) for fiber improvement in the A subgenome and for stress tolerance in the D subgenome. This draft genome sequence provides a resource for engineering superior cotton lines.

altmetric score

  • 38.35

author list (cited authors)

  • Zhang, T., Hu, Y., Jiang, W., Fang, L., Guan, X., Chen, J., ... Chen, Z. J.

citation count

  • 671

publication date

  • January 1, 2015 11:11 AM

keywords

  • Base Sequence
  • Chromosome Mapping
  • Cotton Fiber
  • Genome, Plant
  • Gossypium
  • High-Throughput Nucleotide Sequencing
  • Plant Proteins
  • Sequence Analysis, DNA
  • Tetraploidy