Fabrication methods for a gel-based separation-free device for whole blood glucose detection. Academic Article

abstract

• In this paper, we describe two fabrication methods (well array-based and biopsy punching-based) of gel disks to construct a gel-based point-of-care (POC) diagnosis device for direct colorimetric measurement of human whole blood glucose without any extra blood separation step. The gel disks are made of Polyethylene glycol (PEG) diacrylate (PEG-DA) containing immobilized glucose colorimetric assay reagents. The performances of three types of PEG-DA gel (molecular weight: 575, 3,400, and 10,000) based sensors as well as the two fabrication methods were investigated.The fabricated devices enabled colorimetric whole blood glucose sensing assay without the need for blood cell separationThe biopsy punching-based gel disk fabrication method provided less variation on the fabricated gel disks.

authors

• Han, Arum

published proceedings

• MethodsX

author list (cited authors)

• Zhang, H., Yang, Y., Dai, J., & Han, A.

citation count

• 0

complete list of authors

• Zhang, Han||Yang, Yongjian||Dai, Jing||Han, Arum

publication date

• January 2021

publisher

• Elsevier  Publisher

published in

• MethodsX  Journal

keywords

• Blood Separation
• Glucose Detection
• Hydrogel
• Point Of Care Diagnosis

PubMed Central ID

• 34434759

Digital Object Identifier (DOI)

• 10.1016/j.mex.2021.101236

start page

• 101236

end page

• 101236

volume

• 8

URL

• http://dx.doi.org/10.1016/j.mex.2021.101236