Identification and Confirmation of Molecular Markers and Orange Flesh Color Associated with Quantitative Trait Loci for Beta-Carotene in Muskmelon Conference Paper uri icon

abstract

  • Our objectives were to identify randomly amplified polymorphic DNA (RAPD) and orange flesh color (WF) markers linked to major quantitative trait loci (QTL) for high beta-carotene content using bulked segregant analysis in an F2 population from the muskmelon (Cucumis melo L.) cross of Sunrise (white flesh color and low beta-carotene) x TAM Uvalde (orange flesh color and high betacarotene) in a greenhouse experiment, and confirm the associations of RAPD and WF markers with QTL for this antioxidant trait in an F2 population from the same cross in a field experiment. Continuous distributions for beta-carotene were observed in the F2 populations indicating quantitative inheritance for the trait. A significant positive correlation was found between beta-carotene and total soluble solids. Five RAPD and WF markers were detected to be significantly associated with QTL for high beta-carotene content in the F2 population of the Sunrise x TAM Uvalde cross in the greenhouse based on simple linear regression. Two unlinked markers associated with QTL were significant in a stepwise multiple regression analysis where the full model explained 58% of the total phenotypic variation for beta-carotene. Two QTL were confirmed in the F2 population of the same cross in the field to be consistently associated with the pro-vitamin A trait. These RAPD and WF markers associated with the beta-carotene QTL, consistently expressed in our populations under greenhouse and field environments, could be at least partially utilized in improving the level of this nutrient in new melon cultivars.

published proceedings

  • II INTERNATIONAL SYMPOSIUM ON HUMAN HEALTH EFFECTS OF FRUITS AND VEGETABLES: FAVHEALTH 2007

author list (cited authors)

  • Napier, A. B., Park, S. O., Hwang, H. Y., & Crosby, K. M.

complete list of authors

  • Napier, AB||Park, SO||Hwang, HY||Crosby, KM

publication date

  • January 1, 2009 11:11 AM