An optimized protocol for the generation of HBV viral antigen-specific T lymphocytes from pluripotent stem cells. Academic Article

abstract

• In Tcell-based cancer immunotherapy, tumor antigen (Ag)-specific CD8+ cytotoxic T lymphocytes (CTLs) can specifically target tumor Ags on malignant cells. This promising approach drove us to adopt this strategy of Tcell transfer (ACT)-based immunotherapy for chronic viral infections. Here, we describe the generation of hepatitis B virus (HBV) Ag-specific CTLs from induced pluripotent stem cells (iPSCs), i.e., iPSC-CTLs. Ag-specific iPSC-CTLs can target HBV Ag+ cells and infiltrate into the liver to suppress HBV replication in a murine model. For complete details on the use and execution of this protocol, please refer to Haque etal. (2020).

authors

• Song, Jianxun

published proceedings

• STAR Protoc

author list (cited authors)

• Haque, M., Xiong, X., Lei, F., Das, J. K., & Song, J.

citation count

• 1

complete list of authors

• Haque, Mohammad||Xiong, Xiaofang||Lei, Fengyang||Das, Jugal Kishore||Song, Jianxun

publication date

• January 2021

publisher

• Elsevier  Publisher

published in

• STAR Protocols  Journal

keywords

• Animals
• Hepatitis B
• Hepatitis B Antigens
• Hepatitis B Virus
• Humans
• Immunology
• Immunotherapy, Adoptive
• Induced Pluripotent Stem Cells
• Mice
• Stem Cells
• T-Lymphocytes, Cytotoxic

Digital Object Identifier (DOI)

• 10.1016/j.xpro.2020.100264

start page

• 100264

end page

• 100264

volume

• 2

issue

• 1

URL

• http://dx.doi.org/10.1016/j.xpro.2020.100264

user-defined tag

• 3 Good Health and Well-Being