High-resolution NMR of encapsulated proteins dissolved in low-viscosity fluids. Academic Article uri icon

abstract

  • The majority of known proteins are too large to be comprehensively examined by solution NMR methods, primarily because they tumble too slowly in solution. Here we introduce an approach to making the NMR relaxation properties of large proteins amenable to modern solution NMR techniques. The encapsulation of a protein in a reverse micelle dissolved in a low-viscosity fluid allows it to tumble as fast as a much smaller protein. The approach is demonstrated and validated with the protein ubiquitin encapsulated in reverse micelles prepared in a variety of alkane solvents.

published proceedings

  • Proc Natl Acad Sci U S A

altmetric score

  • 3

author list (cited authors)

  • Wand, A. J., Ehrhardt, M. R., & Flynn, P. F.

citation count

  • 116

complete list of authors

  • Wand, AJ||Ehrhardt, MR||Flynn, PF

publication date

  • December 1998