Optimized reverse micelle surfactant system for high-resolution NMR spectroscopy of encapsulated proteins and nucleic acids dissolved in low viscosity fluids. Academic Article uri icon

abstract

  • An optimized reverse micelle surfactant system has been developed for solution nuclear magnetic resonance studies of encapsulated proteins and nucleic acids dissolved in low viscosity fluids. Comprising the nonionic 1-decanoyl-rac-glycerol and the zwitterionic lauryldimethylamine-N-oxide (10MAG/LDAO), this mixture is shown to efficiently encapsulate a diverse set of proteins and nucleic acids. Chemical shift analyses of these systems show that high structural fidelity is achieved upon encapsulation. The 10MAG/LDAO surfactant system reduces the molecular reorientation time for encapsulated macromolecules larger than ~20 kDa leading to improved overall NMR performance. The 10MAG/LDAO system can also be used for solution NMR studies of lipid-modified proteins. New and efficient strategies for optimization of encapsulation conditions are described. 10MAG/LDAO performs well in both the low viscosity pentane and ultralow viscosity liquid ethane and therefore will serve as a general surfactant system for initiating solution NMR studies of proteins and nucleic acids.

published proceedings

  • J Am Chem Soc

author list (cited authors)

  • Dodevski, I., Nucci, N. V., Valentine, K. G., Sidhu, G. K., O'Brien, E. S., Pardi, A., & Wand, A. J.

citation count

  • 46

complete list of authors

  • Dodevski, Igor||Nucci, Nathaniel V||Valentine, Kathleen G||Sidhu, Gurnimrat K||O'Brien, Evan S||Pardi, Arthur||Wand, A Joshua

publication date

  • February 2014