Dynamic activation and regulation of the mitogen-activated protein kinase p38. Academic Article uri icon

abstract

  • Mitogen-activated protein kinases, which include p38, are essential for cell differentiation and autophagy. The current model for p38 activation involves activation-loop phosphorylation with subsequent substrate binding leading to substrate phosphorylation. Despite extensive efforts, the molecular mechanism of activation remains unclear. Here, using NMR spectroscopy, we show how the modulation of protein dynamics across timescales activates p38. We find that activation-loop phosphorylation does not change the average conformation of p38; rather it quenches the loop ps-ns dynamics. We then show that substrate binding to nonphosphorylated and phosphorylated p38 results in uniform s-ms backbone dynamics at catalytically essential regions and across the entire molecule, respectively. Together, these results show that phosphorylation and substrate binding flatten the energy landscape of the protein, making essential elements of allostery and activation dynamically accessible. The high degree of structural conservation among ser/thr kinases suggests that elements of this mechanism may be conserved across the kinase family.

published proceedings

  • Proc Natl Acad Sci U S A

altmetric score

  • 71.83

author list (cited authors)

  • Kumar, G. S., Clarkson, M. W., Kunze, M., Granata, D., Wand, A. J., Lindorff-Larsen, K., Page, R., & Peti, W.

citation count

  • 39

complete list of authors

  • Kumar, Ganesan Senthil||Clarkson, Michael W||Kunze, Micha BA||Granata, Daniele||Wand, A Joshua||Lindorff-Larsen, Kresten||Page, Rebecca||Peti, Wolfgang

publication date

  • May 2018