Anti-obesity and Anti-inflammatory Effect of Acai Polyphenols in 3T3-L1 Adipocytes Academic Article uri icon

abstract

  • Many health benefits have been attributed to polyphenolics from acai, however, proclaimed antiobesity activities have not yet been investigated in much depth. The aim of this study was to determine the antiobesity and antiinflammatory effects of acai polyphenols (AP) in 3T3L1 mouse adipocytes. Cells were differentiated in the presence of AP at 2.5, 5 and 10 g gallic acid equivalents (GAE)/mL. The activities of AP against lipid synthesis and inflammation were evaluated using RTPCR, Western blotting, and multiplex beadbased immunoassay. Results show that AP reduced the accumulation of intracellular lipids in a dosedependent manner and suppressed mRNA and protein levels of PPAR2, a master regulator of fat synthesis and inflammation to 71% of the control. This was accompanied by downregulation of PPAR2 upstream and downstream genes (C/ebp , C/ebp , Klf5, Srebp1c), PPAR2target adipogenic and proinflammatory adipokine genes. AP also protected against TNFinduced ROS and inflammatory nuclear factor kappa B (NFB), and the NFBtarget genes (interleukins (IL)6, IL1, tumor necrosis factor (TNF), interferon (IFN) and monocyte chemotactic protein1 (MCP1)). These results suggest that AP might be effective in the prevention of obesity and protect against obesityinduced inflammation.

published proceedings

  • FASEB JOURNAL

author list (cited authors)

  • Martino, H., Noratto, G., Dias, M., Talcott, S., & Mertens-Talcott, S.

citation count

  • 0

complete list of authors

  • Martino, Hercia||Noratto, Giuliana||Dias, Manoela||Talcott, Stephen||Mertens-Talcott, Susanne

publication date

  • January 2013

publisher