Short communication: Reproduction outcomes in dairy heifers following a 14-d progesterone insert presynchronization protocol. Academic Article uri icon


  • The objectives were to evaluate pregnancy per artificial insemination (AI), days to first AI, and proportion pregnant within 7 d of AI eligibility in dairy heifers subjected to presynchronization compared with dairy heifers not presynchronized. Thirty days before AI eligibility, Holstein heifers were assigned randomly to 1 of 3 groups: 14-d controlled internal drug release (CIDR; containing progesterone) presynchronization, PGF2 presynchronization, or control (no presynchronization). Heifers in the 14-d CIDR presynchronization treatment (n = 119) received a CIDR on d -30, which was removed on d -16, followed by an injection of PGF2 upon entry to the breeding program (d 0). Heifers in the PGF2 presynchronization treatment (n = 118) received an injection of PGF2 on d -11 and d 0. Control heifers (n = 121) were not presynchronized and received an injection of PGF2 on d 0. All heifers received tail paint on d 0 to facilitate once-daily detection of estrus (based on paint removal). Heifers detected in estrus received AI with conventional semen on the same morning as detected estrus. Generalized linear mixed models were used to assess mean treatment differences. Following PGF2 treatment on d 0, more heifers were detected in estrus in the first 7 d after eligibility in the 14-d CIDR group (95.8%) compared with the PGF2 (74.6%) and control (66.9%) groups. Days to first AI differed between treatments (14-d CIDR = 3.6 d vs. PGF2 = 5.0 d vs. control = 6.8 d). Pregnancy per AI for first AI within 7 d of eligibility was 71.9% (14-d CIDR), 58.0% (PGF2), and 61.7% (control), and differed between 14-d CIDR and PGF2 heifers. Presynchronization with a 14-d CIDR increased the proportion of heifers pregnant in the first 7 d of eligibility (14-d CIDR = 68.9% vs. PGF2 = 43.2% vs. control = 41.3%). Projected days on feed (d 0 to projected calving date) were 295 (14-d CIDR), 302 (PGF2), and 305 (control), and were different between the 14-d CIDR and control heifers. The potential economic benefit to the producer was $15.85 per heifer presynchronized with a 14-d CIDR protocol compared with the control group. Treatment of dairy heifers with a 14-d CIDR effectively presynchronized estrus, resulting in a greater proportion detected in estrus, reduced days to first AI, and an increased proportion of heifers pregnant within the first 7 d after breeding eligibility compared with heifers presynchronized with a single PGF2 injection and control heifers.

published proceedings

  • J Dairy Sci

author list (cited authors)

  • Claypool, C. K., Spencer, J. A., Zoca, S. M., Shafii, B., Price, W. J., Ahmadzadeh, A., Rimbey, N. R., & Dalton, J. C.

citation count

  • 2

complete list of authors

  • Claypool, CK||Spencer, JA||Zoca, S Menegatti||Shafii, B||Price, WJ||Ahmadzadeh, A||Rimbey, NR||Dalton, JC