Chapter Eight Cytoskeletal Proteins at the Lipid Membrane
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The interface at the cell membrane and cytosol offers a wealth of possibilities for intermolecular interactions. Molecular anchors, -bridges, -transmembrane connectors as well as cascades of proteins inside the cell regulate the bidirectional exchange of information between the cell and extracellular environment. Previously, little attention has been given to lipids that are essential for the membrane architecture and for the regulation and function of membrane-associated cytoskeletal proteins. The emergence of new biophysical techniques has spurred rapid acceleration in the ability of researchers to investigate and understand proteinlipid membrane interactions in artificial systems as well as in cells. Stopped-flow kinetics, differential scanning calorimetry (DSC), solid-state nuclear magnetic resonance (NMR) spectroscopy as well as fluorescence recovery after photobleaching (FRAP) will be described and their application will be discussed.