New approaches for understanding the nuclear force balance in living, adherent cells. Academic Article

abstract

• Cytoskeletal forces are transmitted to the nucleus to position and shape it. Linkages mediated by the LINC (linker of nucleoskeleton and cytoskeleton) complex transfer these forces to the nuclear envelope. Nuclear position and shape can be thought to be determined by a balance of cytoskeletal forces generated by microtubule motors that shear the nuclear surface, actomyosin forces that can pull, push and shear the nucleus, and intermediate filaments that may passively resist nuclear decentering and deformation. Parsing contributions of these different forces to nuclear mechanics is a very challenging task. Here we review new approaches that can be used in living cells to probe and understand the nuclear force balance.

authors

• Lele, Tanmay

published proceedings

• Methods

author list (cited authors)

• Neelam, S., Dickinson, R. B., & Lele, T. P.

citation count

• 4

complete list of authors

• Neelam, Srujana||Dickinson, Richard B||Lele, Tanmay P

publication date

• February 2016

publisher

• Elsevier  Publisher

published in

• Methods  Journal

keywords

• Cell Adhesion
• Cell Movement
• Cell Nucleus
• Cytoskeleton
• Humans
• Linc Complex
• Microscopy, Atomic Force
• Nuclear Forces
• Nuclear Position
• Nuclear Shape

PubMed Central ID

• 26115785

Digital Object Identifier (DOI)

• 10.1016/j.ymeth.2015.06.014

start page

• 27

end page

• 32

volume

• 94

URL

• http://dx.doi.org/10.1016/j.ymeth.2015.06.014