Investigation of in vivo microtubule and stress fiber mechanics with laser ablation. Academic Article

abstract

• Laser ablation has emerged as a useful technique to study the mechanical properties of the cytoskeleton in living cells. Laser ablation perturbs the force balance in the cytoskeleton, resulting in a dynamic response which can be imaged. Quantitative measurement of the dynamic response allows the testing of mechanical theories of the cytoskeleton in living cells. This review discusses recent work in applying laser ablation to study stress fiber and microtubule mechanics in living endothelial cells. These studies reveal that molecular motors are major determinants of the mechanical properties of the cytoskeleton in cells.

authors

• Lele, Tanmay

published proceedings

• Integr Biol (Camb)

author list (cited authors)

• Wu, J., Dickinson, R. B., & Lele, T. P.

citation count

• 12

complete list of authors

• Wu, Jun||Dickinson, Richard B||Lele, Tanmay P

publication date

• May 2012

publisher

• Oxford University Press (OUP)  Publisher

published in

• Integrative Biology: interdisciplinary approaches for molecular and cellular life sciences  Journal

keywords

• Animals
• Cytoskeleton
• Humans
• Laser Capture Microdissection
• Laser Therapy
• Microtubules
• Molecular Motor Proteins
• Stress Fibers

PubMed Central ID

• 22495508

Digital Object Identifier (DOI)

• 10.1039/c2ib20015e

start page

• 471

end page

• 479

volume

• 4

issue

• 5

URL

• http://dx.doi.org/10.1039/c2ib20015e