Stamp wound assay for studying coupled cell migration and cell debris clearance. Academic Article

abstract

• A new method for studying wound healing under realistic conditions in vitro was developed. The method involves creating defined patterns of damaged cell debris with poly(dimethyl)siloxane (PDMS) stamping. This novel assay permitted the quantification of wound healing rates in the presence of cell debris. Experimental results with this assay suggest that cell migration in the presence of cell debris is a two step process requiring (1) non-muscle myosin II-dependent cell clearance followed by (2) cell migration into newly cleared wound areas. The novel stamp wound assay allows the study of coupled cell migration and debris clearance and is a more realistic wound healing assay in vitro.

authors

• Lele, Tanmay

published proceedings

• Langmuir

author list (cited authors)

• Lee, J., Wang, Y., Ren, F., & Lele, T. P.

citation count

• 19

complete list of authors

• Lee, Jiyeon||Wang, Yu-Lin||Ren, Fan||Lele, Tanmay P

publication date

• November 2010

publisher

• American Chemical Society (ACS)  Publisher

published in

• Langmuir  Journal

keywords

• Cell Death
• Cell Line
• Cell Movement
• Cells, Immobilized
• Dimethylpolysiloxanes
• Epithelial Cells
• Heterocyclic Compounds, 4 Or More Rings
• Humans
• Myosin Type Ii
• Time Factors
• Wound Healing

PubMed Central ID

• 20961056

Digital Object Identifier (DOI)

• 10.1021/la103542y

start page

• 16672

end page

• 16676

volume

• 26

issue

• 22

URL

• http://dx.doi.org/10.1021/la103542y