Trump, Bryan Glen (2014-05). Periostin and Osterix Expression in Odontogenic and Non-Odontogenic Bone Lesions. Master's Thesis. Thesis uri icon

abstract

  • Osterix is a transcription factor that is essential for osteoblast differentiation and periostin is a matricellular protein that, in the jaw bones, is expressed in the periosteum and periodontal ligament (PDL). It has been hypothesized that the co-expression of osterix and periostin supports a histogenesis of PDL origin. The purpose of this study was to investigate the expression patterns of periostin and osterix in some jawbone lesions and correlate the results with current concepts regarding the cell of origin of these bone diseases. We also wanted to test whether or not the co-expression of periostin and osterix are indeed PDL- specific. We investigated the expression patterns of periostin and osterix, using immunohistochemistry, in cases of cemento-osseous dysplasia (COD), ossifying fibroma of the jawbones (OFJ), osteoblastoma of the jawbones (OBJ), odontogenic myxoma (OM), osteosarcoma of the jawbones (OSJ), osteosarcoma of the long bones (OSL) and osteoblastoma of the long bones (OBL). All bone lesions (n=57) selected for this study stained positive for periostin. For osterix expression, COD, OSJ, OBL, and most cases of OFJ, OM and OBJ showed positive results. OSL, except for the anaplastic/fibroblastic histological variant, also showed osterix expression. As there is no PDL in the long bones, these results demonstrated that the co-expression of these two proteins was not PDL-specific. The co-expression of osterix and periostin in OM lesional cells suggest a potential differentiation for osteogenesis or PDL origin, despite no calcified material is found in this neoplasm. We also report a novel finding that osterix-positive cells and periostin expression were found in the lamina propria, and osterix-positive cells were also found in squamous epithelium of normal gingival mucosa.
  • Osterix is a transcription factor that is essential for osteoblast differentiation and
    periostin is a matricellular protein that, in the jaw bones, is expressed in the periosteum
    and periodontal ligament (PDL). It has been hypothesized that the co-expression of
    osterix and periostin supports a histogenesis of PDL origin. The purpose of this study
    was to investigate the expression patterns of periostin and osterix in some jawbone
    lesions and correlate the results with current concepts regarding the cell of origin of
    these bone diseases. We also wanted to test whether or not the co-expression of
    periostin and osterix are indeed PDL- specific. We investigated the expression patterns
    of periostin and osterix, using immunohistochemistry, in cases of cemento-osseous
    dysplasia (COD), ossifying fibroma of the jawbones (OFJ), osteoblastoma of the
    jawbones (OBJ), odontogenic myxoma (OM), osteosarcoma of the jawbones (OSJ),
    osteosarcoma of the long bones (OSL) and osteoblastoma of the long bones (OBL). All
    bone lesions (n=57) selected for this study stained positive for periostin. For osterix
    expression, COD, OSJ, OBL, and most cases of OFJ, OM and OBJ showed positive
    results. OSL, except for the anaplastic/fibroblastic histological variant, also showed
    osterix expression. As there is no PDL in the long bones, these results demonstrated that
    the co-expression of these two proteins was not PDL-specific. The co-expression of
    osterix and periostin in OM lesional cells suggest a potential differentiation for
    osteogenesis or PDL origin, despite no calcified material is found in this neoplasm. We
    also report a novel finding that osterix-positive cells and periostin expression were found
    in the lamina propria, and osterix-positive cells were also found in squamous epithelium of normal gingival mucosa.

publication date

  • May 2014