Construction of bacterial artificial chromosome libraries for Zhikong Scallop Chlamys farreri Academic Article uri icon

abstract

  • Two Large-insert genomic bacterial artificial chromosome (BAC) libraries of Zhikong scallop Chlamys farreri were constructed to promote our genetic and genomic research. High-quality megabase-sized DNA was isolated from the adductor muscle of the scallop and partially digested by BamH I and Mbo I, respectively. The BamH I library consisted of 53 760 clones while the Mbo I library consisted of 7 680clones. Approximately 96 % of the clones in BamH I library contained nuclear DNA inserts in average size of 100 kb, providing a coverage of 5. 3 haploid genome equivalents. Similarly, the Mbo I library with an average insert of 145 kb and no insert-empty clones, thus providing a genome coverage of 1. 1 haploid genome equivalents. 2008 Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg.

published proceedings

  • CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY

author list (cited authors)

  • Zhang, Y., Zhang, X., Scheuring, C. E., Zhang, H., Li, F., & Xiang, J.

complete list of authors

  • Zhang, Yang||Zhang, Xiaojun||Scheuring, Chantel E||Zhang, Hongbin||Li, Fuhua||Xiang, Jianhai

publication date

  • January 1, 2008 11:11 AM