Serum biochemical profile in buffalo endometritis and impact of treatment with PGF2 and intrauterine gentamicin infusion on postpartum reproductive performance. Academic Article uri icon


  • Improving reproductive performance of repeat breeder buffalo cows due to clinical endometritis is crucial in overcoming infertility problems in buffalo cows. The aim of the present study was to use PGF2 and/or gentamicin 10% for treatment of endometritis and to determine biochemical parameters in serum that could be used to diagnose endometritis in buffalo cows. A total of 64 anestrous buffalo cows were assigned into one of five treatment groups: group one (n=9) buffalo cows were physiological normal cows and served as a control group; group 2 buffalo cows had endometritis, but were not treated (n=10); group 3 buffalo cows had endometritis and were treated with intrauterine delivery of 100ml of a 10% gentamicin sulfate solution in three times within 1week (n=15); group 4 buffalo cows received two I.M. doses of PGF2 (2ml Estrumate IM) at the time of corpus luteum dominance (n=15) (treatment 4 does not make sense as you stated that the cows were in anestrus) on the ovary; group 5 buffalo cows received two IM doses of PGF2 at an 11day interval and the 10% gentamicin solution as described for group 3. Serum samples were collected from control, pre-treated, and post-treated buffalo cows with endometritis to evaluate the diagnostic biochemical parameters. The days to first estrus (DFE), number of services per conception (S/C), days open (DO), and pregnancy rate (Preg) were the measures for determining reproductive performance for the buffalo cows. The buffalo cows treated by gentamicin and PGF2 exhibited their first estrus earlier than cows in the other four groups of cows. The number of days open for control cows was greater (P<0.05) than for the other groups and the control cows, as were the number of services per conception (P<0.05) and pregnancy rate (30%: P<0.05). The receiver operating characteristic (ROC) curve was used to identify biochemical parameters in serum to predict endometritis. Creatine kinase (CK), aspartate aminotransferase (AST), and concentrations of total bilirubin and immunoglobulins in serum were greater (P<0.05), while serum albumin values were lower (P<0.05) in serum of buffalo cows with endometritis. From the ROC analyses, CK was the most predictable biomarker for endometritis with an area under the curve of 0.889, sensitivity of 80%, and specificity of 100% (P<0.001). In conclusion, the use of gentamicin and PGF2 for treatment of endometritis improves the reproductive performance of buffalo cows, and concentrations of CK serve as an aid for diagnosing endometritis.Graphical abstract.

published proceedings

  • Trop Anim Health Prod

altmetric score

  • 1

author list (cited authors)

  • Elmetwally, M. A., Elshopakey, G. E., El-Desouky, A. M., Eldomany, W. B., & Bazer, F. W.

citation count

  • 1

complete list of authors

  • Elmetwally, Mohammed Ahmed||Elshopakey, Gehad E||El-Desouky, Ashraf M||Eldomany, Wael B||Bazer, Fuller W

publication date

  • November 2020