Discovery of Potent Charge-Reducing Molecules for Native Ion Mobility Mass Spectrometry Studies.
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There is growing interest in the characterization of protein complexes and their interactions with ligands using native ion mobility mass spectrometry. A particular challenge, especially for membrane proteins, is preserving noncovalent interactions and maintaining native-like structures. Different approaches have been developed to minimize activation of protein complexes by manipulating charge on protein complexes in solution and the gas-phase. Here, we report the utility of polyamines that have exceptionally high charge-reducing potencies with some molecules requiring 5-fold less than trimethylamine oxide to elicit the same effect. The charge-reducing molecules do not adduct to membrane protein complexes and are also compatible with ion-mobility mass spectrometry, paving the way for improved methods of charge reduction.
author list (cited authors)
Lyu, J., Liu, Y., McCabe, J. W., Schrecke, S., Fang, L., Russell, D. H., & Laganowsky, A.
complete list of authors
Lyu, Jixing||Liu, Yang||McCabe, Jacob W||Schrecke, Samantha||Fang, Lei||Russell, David H||Laganowsky, Arthur