Discovery of Potent Charge-Reducing Molecules for Native Ion Mobility Mass Spectrometry Studies. Academic Article uri icon


  • There is growing interest in the characterization of protein complexes and their interactions with ligands using native ion mobility mass spectrometry. A particular challenge, especially for membrane proteins, is preserving noncovalent interactions and maintaining native-like structures. Different approaches have been developed to minimize activation of protein complexes by manipulating charge on protein complexes in solution and the gas-phase. Here, we report the utility of polyamines that have exceptionally high charge-reducing potencies with some molecules requiring 5-fold less than trimethylamine oxide to elicit the same effect. The charge-reducing molecules do not adduct to membrane protein complexes and are also compatible with ion-mobility mass spectrometry, paving the way for improved methods of charge reduction.

published proceedings

  • Anal Chem

altmetric score

  • 5.6

author list (cited authors)

  • Lyu, J., Liu, Y., McCabe, J. W., Schrecke, S., Fang, L., Russell, D. H., & Laganowsky, A.

citation count

  • 15

complete list of authors

  • Lyu, Jixing||Liu, Yang||McCabe, Jacob W||Schrecke, Samantha||Fang, Lei||Russell, David H||Laganowsky, Arthur

publication date

  • August 2020