QUANTITATION OF HYMENOXON AND RELATED SESQUITERPENE LACTONES Academic Article uri icon

abstract

  • A rapid and reproducible method for the quantitation of hymenoxon and related sesquiterpene lactones has been developed using reversed-phase HPLC. The methanol extract of a dried and ground plant sample was analyzed on an RP-8 column eluted with methanol-water (1:1, v/v) and monitored at 235 nm. The hymenoxon content of the above ground whole plant of Hymenoxys odorata ranged from 0.21 to 1.27% according to the site, season, and year of collection. Helenalin and mexicanin E were concentrated in the flowering heads (8.95 and 4.66%, respectively) of Helenium microcephalum while tenulin was concentrated in the leaves (6.14%) of Helenium amarum. The detection limit was 25 ng for helenalin and mexicanin E and 75 ng for hymenoxon and tenulin. The recovery rate of hymenoxon was 102.5 4.5%. 1987, American Chemical Society. All rights reserved.

published proceedings

  • JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

author list (cited authors)

  • KIM, H. L., SAFE, S. H., & CALHOUN, M. C.

citation count

  • 6

complete list of authors

  • KIM, HL||SAFE, SH||CALHOUN, MC

publication date

  • November 1987