QUANTITATION OF HYMENOXON AND RELATED SESQUITERPENE LACTONES

abstract

• A rapid and reproducible method for the quantitation of hymenoxon and related sesquiterpene lactones has been developed using reversed-phase HPLC. The methanol extract of a dried and ground plant sample was analyzed on an RP-8 column eluted with methanol-water (1:1, v/v) and monitored at 235 nm. The hymenoxon content of the above ground whole plant of Hymenoxys odorata ranged from 0.21 to 1.27% according to the site, season, and year of collection. Helenalin and mexicanin E were concentrated in the flowering heads (8.95 and 4.66%, respectively) of Helenium microcephalum while tenulin was concentrated in the leaves (6.14%) of Helenium amarum. The detection limit was 25 ng for helenalin and mexicanin E and 75 ng for hymenoxon and tenulin. The recovery rate of hymenoxon was 102.5 4.5%. 1987, American Chemical Society. All rights reserved.

authors

• Safe, Stephen

published proceedings

• JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

author list (cited authors)

• KIM, H. L., SAFE, S. H., & CALHOUN, M. C.

citation count

• 6

complete list of authors

• KIM, HL||SAFE, SH||CALHOUN, MC

publication date

• November 1987

publisher

• American Chemical Society (ACS)  Publisher

published in

• Journal of Agricultural and Food Chemistry  Journal

Digital Object Identifier (DOI)

• 10.1021/jf00078a010

start page

• 891

end page

• 895

volume

• 35

issue

• 6

URL

• http://dx.doi.org/10.1021/jf00078a010