De novo biosynthesis of terminal alkyne-labeled natural products. Academic Article

abstract

• The terminal alkyne is a functionality widely used in organic synthesis, pharmaceutical science, material science and bioorthogonal chemistry. This functionality is also found in acetylenic natural products, but the underlying biosynthetic pathways for its formation are not well understood. Here we report the characterization of what is to our knowledge the first carrier protein-dependent terminal alkyne biosynthetic machinery in microbes. We further demonstrate that this enzymatic machinery can be exploited for the in situ generation and incorporation of terminal alkynes into two natural product scaffolds in Escherichia coli. These results highlight the prospect for tagging major classes of natural products, including polyketides and polyketide/nonribosomal peptide hybrids, using biosynthetic pathway engineering.

authors

• Zhu, Xuejun

published proceedings

• Nat Chem Biol

altmetric score

• 4.25

author list (cited authors)

• Zhu, X., Liu, J., & Zhang, W.

citation count

• 94

complete list of authors

• Zhu, Xuejun||Liu, Joyce||Zhang, Wenjun

publication date

• February 2015

publisher

• Springer Nature  Publisher

published in

• Nature Chemical Biology  Journal

keywords

• Alkynes
• Bacterial Proteins
• Biological Products
• Biosynthetic Pathways
• Escherichia Coli
• Molecular Sequence Data
• Organisms, Genetically Modified
• Peptide Biosynthesis, Nucleic Acid-independent
• Polyketides

PubMed Central ID

• 25531891

Digital Object Identifier (DOI)

• 10.1038/nchembio.1718

start page

• 115

end page

• 120

volume

• 11

issue

• 2

URL

• http://dx.doi.org/10.1038/nchembio.1718