A fluorogenic screening platform enables directed evolution of an alkyne biosynthetic tool. Academic Article uri icon

abstract

  • Directed evolution was used to improve the activity of JamB, a membrane-bound bifunctional desaturase/acetylenase. To quickly assess the protein engineering outcomes, we developed a new platform for quantifying extracellular alkyne-tagged metabolites through a fluorogenic click reaction. Random mutagenesis yielded the best JamB variant with ∼20-fold increased activity in E. coli.

published proceedings

  • Chem Commun (Camb)

altmetric score

  • 0.25

author list (cited authors)

  • Zhu, X., Shieh, P., Su, M., Bertozzi, C. R., & Zhang, W.

citation count

  • 17

complete list of authors

  • Zhu, Xuejun||Shieh, Peyton||Su, Michael||Bertozzi, Carolyn R||Zhang, Wenjun

publication date

  • September 2016