A fluorogenic screening platform enables directed evolution of an alkyne biosynthetic tool Academic Article uri icon

abstract

  • Directed evolution was used to improve the activity of JamB, a membrane-bound bifunctional desaturase/acetylenase. To quickly assess the protein engineering outcomes, we developed a new platform for quantifying extracellular alkyne-tagged metabolites through a fluorogenic click reaction. Random mutagenesis yielded the best JamB variant with ∼20-fold increased activity in E. coli.

altmetric score

  • 0.25

author list (cited authors)

  • Zhu, X., Shieh, P., Su, M., Bertozzi, C. R., & Zhang, W.

citation count

  • 13

publication date

  • September 2016