A fluorogenic screening platform enables directed evolution of an alkyne biosynthetic tool. Academic Article

abstract

• Directed evolution was used to improve the activity of JamB, a membrane-bound bifunctional desaturase/acetylenase. To quickly assess the protein engineering outcomes, we developed a new platform for quantifying extracellular alkyne-tagged metabolites through a fluorogenic click reaction. Random mutagenesis yielded the best JamB variant with 20-fold increased activity in E. coli.

authors

• Zhu, Xuejun

published proceedings

• Chem Commun (Camb)

altmetric score

• 0.25

author list (cited authors)

• Zhu, X., Shieh, P., Su, M., Bertozzi, C. R., & Zhang, W.

citation count

• 17

complete list of authors

• Zhu, Xuejun||Shieh, Peyton||Su, Michael||Bertozzi, Carolyn R||Zhang, Wenjun

publication date

• September 2016

publisher

• Royal Society of Chemistry (RSC)  Publisher

published in

• Chemical Communications  Journal

keywords

• 0601 Biochemistry And Cell Biology

PubMed Central ID

• 27561030

Digital Object Identifier (DOI)

• 10.1039/c6cc05990b

start page

• 11239

end page

• 11242

volume

• 52

issue

• 75