High-throughput mutagenized cell screening system capable of selective single cell extraction
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We have developed a high-throughput microfluidic screening platform capable of culturing, analyzing, and selectively extracting mutagenized cells to investigate genetic variants. Combination of two pneumatic control layers, each comprising 32 microchannels, enables individually addressing and controlling each of the 1024 trapping sites in the cell analysis layer. By pressurizing either of the two pneumatic control channels or releasing pressure from both channels, the underlying trapping site was opened or closed, respectively. Cells inside a particular trapping site were extracted with backflow by selectively opening only this site. The performance of this screening platform was characterized using green microalga Tetraslimis suecica.