Regulation of Junonia coenia densovirus P9 promoter expression. Academic Article

abstract

• Transcriptional activity of the Junonia coenia densovirus (JcDNV) P9 promoter depends on a 557-bp sequence located within the overlapping 3' sequences for viral capsid and nonstructural genes. Utilizing a somatic transformation assay to assess JcDNV promoter activity in Drosophila melanogaster and Plodia interpunctella, viral sequences were subjected to deletional analysis. Removal of a 685-bp fragment reduced P9-driven expression to background levels. Inclusion of a second expression cassette demonstrated vector persistence and confirmed somatic transformation. P9 promoter-driven expression was restored by insertion of a 557-bp JcDNV fragment or by inclusion of a heterologous baculovirus hr5 enhancer. Consensus polycomb transcriptional factor binding sites were identified within the 557-bp fragment, which suggests a potential role in regulating densoviral transcription.

authors

• Gillett-Kaufman, Jennifer

published proceedings

• Insect Mol Biol

author list (cited authors)

• Shirk, P. D., Bossin, H., Furlong, R. B., & Gillett, J. L.

citation count

• 8

complete list of authors

• Shirk, PD||Bossin, H||Furlong, RB||Gillett, JL

publication date

• October 2007

publisher

• Wiley  Publisher

published in

• INSECT MOLECULAR BIOLOGY  Journal

keywords

• Animals
• Base Sequence
• Butterflies
• Capsid Proteins
• Densovirus
• Gene Expression Regulation, Viral
• Genes, Viral
• Genetic Vectors
• Molecular Sequence Data
• Plasmids
• Promoter Regions, Genetic
• Sequence Deletion
• Transformation, Genetic
• Viral Nonstructural Proteins

PubMed Central ID

• 17714462

Digital Object Identifier (DOI)

• 10.1111/j.1365-2583.2007.00759.x

start page

• 623

end page

• 633

volume

• 16

issue

• 5

URL

• http://dx.doi.org/10.1111/j.1365-2583.2007.00759.x