Virus-Induced Gene Silencing in Diploid and Tetraploid Potato Species. Academic Article

abstract

• Potato is the world's fourth largest food crop and a vegetatively propagated model polyploid plant. To facilitate genomic studies in potato, here we describe detailed protocols to silence genes in both diploid potato Solanum bulbocastanum and tetraploid potato cultivars such as Maris Bard, Arran Pilot, Ancilla, and Serrana using tobacco rattle virus (TRV)- or potato virus X(PVX)-induced gene silencing (VIGS) system, respectively. The established VIGS system represents an efficient and powerful approach for functional analysis of genes involved in growth, development, metabolism, and responses to biotic and abiotic stresses in potato.

authors

• Song, Junqi

published proceedings

• Methods Mol Biol

altmetric score

• 0.75

author list (cited authors)

• Zhao, J., Jiang, H., Wang, G., Wang, Z., Dong, J., & Song, J.

citation count

• 2

complete list of authors

• Zhao, Jinping||Jiang, Haolang||Wang, Guanyu||Wang, Zonghua||Dong, Jingao||Song, Junqi

publication date

• January 2020

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Diploid Potato
• Diploidy
• Gene Expression Regulation, Plant
• Gene Silencing
• Plant Diseases
• Plant Viruses
• Potato Virus X (pvx )
• Potexvirus
• Solanum Bulbocastanum
• Solanum Tuberosum
• Tetraploid Potato
• Tetraploidy
• Tobacco
• Tobacco Rattle Virus (trv )
• Virus-induced Gene Silencing (vigs )

Digital Object Identifier (DOI)

• 10.1007/978-1-0716-0751-0_4

start page

• 39

end page

• 50

volume

• 2172

URL

• http://dx.doi.org/10.1007/978-1-0716-0751-0_4