Genetic Predisposition To The Expression Of Lymphoid Procoagulant Activity (PCA) In Response To Viral Stimulation Conference Paper uri icon

abstract

  • A predisposing foctor in the induction of viral induced disease may be the genetic make-up of the host. Leukocytes from a variety of species uniformly generate increased amounts of procoagulant molecules in response to a variety of stimuli including antigen:antibody complexes, lipopolysaccharides (LPS), complement fragments C5a and lectins. Although the amount of increased PCA varies with the concentration and duration of contact with the stimulus, all individuals respond to equivalently these stimuli. The coronavirus family is a group of RNA viruses and includes the murine hepatitis virus (MHV) which causes strain dependent forms of disease in mice. BaIb/C and DBA/2 mice develop acute necrotizing hepatitis, C3H mice develop a mild chronic disease and the A strain develops no evidence of hepatitis. Peripheral blood mononuclear cells from BaIb/C and DBA/2 mice respond both in vitro and in vivo with a marked increase in procoagulant activity (PCA), with 106 PFU of MHV-3 invoking an 80-fold increase in PCA and 10 PFU a 10 fold increase in PCA. PBM from C3H strain mice respond to MHV with a 6.8-fold increase of PCA at 106 PFU of virus. In constrast, PBM from A strain mice do not respond to 10-106 PFU or virus. The PCA was a product of monocytes as demonstrated by direct cytologic assay, however induction of PCA required the presence of lymphocytes. Two types of PCA were found, an early increase of a novel cellular prothrombinase and later increase in thromboplastin activity. Thus, the expression of MHV induced PCA appears to be genetically restricted and also correlates with susceptibility to viral induction of disease. The existence of comparable genetic restrictions in man might relate the variable incidence of thrombotic diseases. Supported by NIH grants HL- 16411 and CA-28166.

name of conference

  • Oral Presentations

published proceedings

  • Thrombosis and Haemostasis

author list (cited authors)

  • Levy, G. A., Leibowitz, J. L., & Edgington, T. S.

citation count

  • 1

complete list of authors

  • Levy, GA||Leibowitz, JL||Edgington, TS

publication date

  • January 1981

publisher