Novel high-throughput screening system for cancer therapy with simultaneous combination treatments
Additional Document Info
In this paper, we report the development of a microfluidic high-throughput screening system for high-throughput screening of drug molecule interactions on cancer cell death. A key feature is that 64 pair-wise concentrations (between eight concentrations of two drugs) are generated using a traditional diffusive microfluidic mixer and a time-dependent variable concentration mixer. Using this novel high-throughput combination array, we generated pair-wise combinations of the cancer drug sensitizer doxorubicin and the cytokine TRAIL, and used it for on-chip screening against PC3 prostate cancer cells. Our results identify a concentration range where doxorubicin and TRAIL are synergistic and demonstrates the utility of the system as a high-throughput anti-cancer drug screening tool.