Skeletal Muscle Anabolic Response Following Incubation with Protein Kinase C Activator OAG
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PurposeFor years, there has been a growing interest in the role of lipids in the progression of type 2 diabetes. Specifically, molecules such as diacylglycerol have garnered attention due to its concomitant increase with the progression of insulin resistance and type 2 diabetes. These experiments aim to further characterize the role of diacylglycerol in cellular metabolism by using the diacylglycerol mimetic, 1oleoyl2acetylsnglycerol (OAG) alongside mTOR inhibitors rapamycin or Torin 1.MethodsC2C12 myoblasts were grown and maintained in 10 cm plates with high glucose DMEM growth media until reaching ~80% confluence, at which point they were transitioned to a high glucose differentiation media to encourage myotube formation. Six days following differentiation, plates were randomly assigned to treatment groups (n=3) and treated in the presence of 4% deuterium oxide for 24 hours. Myotubes were harvested with cell scrapers in the presence of Norris lysis buffer, and cellular contents were fractionated for measures of both protein synthesis rates and anabolic signaling via western blot analysis.ResultsPresence of OAG (20 M) did not significantly alter 24 hour rates of protein synthesis (1.728%/hr) compared to controls (1.630%/hr) (p = 1.000). Rapamycin (1.333%/hr) and Torin 1 (1.187%/hr) treatments significantly reduced protein synthesis rates compared to controls (p = 0.003 and p < 0.001 respectively). However, OAG did not have any statistically significant effect on mitigating the suppressive effects of rapamycin (OAG+Rapamycin 1.380%/hr) (p=1.000) or Torin 1 (OAG+Torin 1 1.158%/hr) (p=1.000).ConclusionsOAG is known to be a potent activator of Protein Kinase C as a cell permeable analog of diacylglycerol. These data indicate that a 24hour incubation with OAG is not sufficient to significantly perturb fractional rates of protein synthesis in murine skeletal muscle myotubes.Support or Funding InformationWork by this author was partially supported by the Sydney & J.L. Huffines Institute for Sports Medicine & Human Performance, and a Texas A&M College of Education and Human Development Graduate Research award.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
