Characterization of poly(amidoamine) dendrimers and their complexes with Cu2+ by matrix-assisted laser desorption ionization mass spectrometry
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abstract
Hydroxyl-terminated poly(amidoamine) (PAMAM) dendrimers of generation 2-4 (Gn-OH, n = 2, 3, and 4; Mr 3.7, 6.9, and 14.3 kDa, respectively) and dendrimers encapsulating Cu2+ ions have been investigated by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. Umbelliferone (7-hydroxycoumarin) and 2, 4,6-trihydroxyacetophenone (THAP) were used as the matrices for dendrimers with and without Cu2+ in the interior. The MALDI-TOF results provide accurate molecular weight determinations of all dendrimers and some multiple copper complexes of G2-OH and G3-OH (accuracy < 0.01%). More importantly, this approach provides direct characterization of multiple-cation adducts and quantitative evaluation of the complexing capacity of dendrimers hosting transition-metal ions. The results are used to determine dendrimer structure and evaluate polydispersity.