Phosphorus-31 NMR relaxation studies of diethyl p-methoxyphenyl phosphate bound to phosphotriesterase
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The effect of Mn2+ Mn2+, Mn2+ Zn2+ and Mn2+ Cd2+ reconstituted phosphotriesterase on the 31P spin lattice ( 1 T1) relaxation rate of diethyl p-methoxyphenyl phosphate has been investigated. In the presence of Mn2+ Mn2+ phosphotriesterase, the spin lattice relaxation rate of the phosphorus atom is enhanced giving an upper limit for the phosphorus-metal root mean-sixth average distance of 4.2 . These results demonstrate for the first time that substrates for phosphotriesterase bind in close proximity to the binuclear metal center. 1995.