Synthesis and enzymatic hydrolysis of a light-emitting substrate for phosphotriesterase
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The diethylphosphate derivative of 4-methoxy-4-(3-hydroxyphenyl)spiro[1,2-dioxetane-3,2′-adamantane] was synthesized. The Km and Vmax values for the enzymatic hydrolysis by phosphotriesterase were 0.091 mM and 0.15 sec-1, respectively. The enzyme-catalyzed hydrolysis was accompanied by the emission of visible light. © 1994.
author list (cited authors)
Hong, S., & Raushel, F. M.