Preparation of 5- and 6-Carboxyfluorescein Academic Article uri icon


  • Condensation of resorcinol with 4-carboxyphthalic anhydride in methanesulfonic acid gave a mixture of 5- and 6-carboxyfluorescein stereoisomers. These were separated by recrystallization from methanol- or ethanol-hexane to give 5- and 6-carboxyfluorescein, each in over 98% purity.

altmetric score

  • 9

author list (cited authors)

  • Ueno, Y., Jiao, G., & Burgess, K.

citation count

  • 15

publication date

  • January 2004