Preparation of 5- and 6-Carboxyfluorescein
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Condensation of resorcinol with 4-carboxyphthalic anhydride in methanesulfonic acid gave a mixture of 5- and 6-carboxyfluorescein stereoisomers. These were separated by recrystallization from methanol- or ethanol-hexane to give 5- and 6-carboxyfluorescein, each in over 98% purity.
author list (cited authors)
Ueno, Y., Jiao, G., & Burgess, K.