SYNTHESIS OF AN OXYAMIDE LINKED NUCLEOTIDE DIMER AND INCORPORATION INTO ANTISENSE OLIGONUCLEOTIDE SEQUENCES
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abstract
Two derivatives of thymidine, a 5-protected 3-hydroxylamine and 5-carboxy-3-protected compound, were prepared and coupled to form an oxyamide-linked dinucleotide analogue; this dimer was incorporated into an oligodeoxynucleotide then shown to anneal to complementary DNA with nearly the same affinity as the natural sequence.