An overview of techniques for immobilizing and viewing living cells.
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Microscopists making observations on living cells are often faced with the challenge of getting those cells to hold still for extended observation times. This paper presents an overview/summary of a range of techniques for non-destructive immobilization of living cells (with an emphasis on protozoa), permitting microscopic observations and photography. A variety of chemical and physical immobilization techniques are discussed, but particular attention is paid to a comparative discussion of the mechanical devices (rotocompressors or microcompressors) used for reversible trapping of living cells.
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