SIZE, CHARGE AND STRUCTURAL HETEROGENEITY OF BRUCELLA-ABORTUS LIPOPOLYSACCHARIDES DEMONSTRATED BY TWO-DIMENSIONAL GEL-ELECTROPHORESIS Academic Article uri icon

abstract

  • Phenol extracted, alkalitreated lipopolysaccharide (aLPS) from vaccine strain (S19) Brucella abortus was demonstrated by twodimensional gel electrophoresis to consist of at least ten silver staining, polydisperse analogues having different pIs. When tested on nitrocellulose immunoblots, all ten were antigenically reactive with bovine antiB. abortus polyclonal sera, but only six reacted with antiB. abortus Oantigen murine monoclonal antibody. Analogues focusing at different pIs were concluded to arise from differences in either core or Oantigen side chain structure or because of covalently bound protein. While not qualitatively different, aLPS from pathogenic B. abortus strain 2308 had lesser amounts of analogues 1, 2, 5, 6, and 8 than did aLPS from strain 19 (vaccine). The 2D gel electrophoresis method was demonstrated to be of value in the analysis of aLPS from B. abortus and may be useful in the study of lipopolysaccharides from other sources. Copyright 1986 VCH Verlagsgesellschaft mbH

published proceedings

  • ELECTROPHORESIS

altmetric score

  • 3

author list (cited authors)

  • SOWA, B. A., CRAWFORD, R. P., HECK, F. C., WILLIAMS, J. D., WU, A. M., KELLY, K. A., & ADAMS, L. G.

citation count

  • 6

complete list of authors

  • SOWA, BA||CRAWFORD, RP||HECK, FC||WILLIAMS, JD||WU, AM||KELLY, KA||ADAMS, LG

publication date

  • January 1986

publisher