Potato Virus X-Based microRNA Silencing (VbMS) In Potato. Academic Article

abstract

• Virus-based microRNA silencing (VbMS) is a rapid and efficient tool for functional characterization of microRNAs (miRNAs) in plants. The VbMS system has been developed and applied for various plant species including Nicotiana benthamiana, tomato, Arabidopsis, cotton, and monocot plants such as wheat and maize. Here, we describe a detailed protocol using PVX-based VbMS vectors to silence endogenous miRNAs in potato. To knock down the expression of a specific miRNA, target mimic (TM) molecules of miRNA of interest are designed, integrated into plant virus vectors, and expressed in potato by Agrobacterium infiltration to bind directly to the endogenous miRNA of interest and block its function.

authors

• Song, Junqi

published proceedings

• J Vis Exp

altmetric score

• 0.75

author list (cited authors)

• Zhao, J., Rios, C. G., & Song, J.

citation count

• 7

complete list of authors

• Zhao, Jinping||Rios, Carlos Garcia||Song, Junqi

publication date

• May 2020

publisher

• MyJove Corporation  Publisher

published in

• Journal of Visualized Experiments  Journal

keywords

• Base Sequence
• Gene Expression Regulation, Plant
• Gene Silencing
• Genetic Vectors
• MicroRNAs
• Potexvirus
• Solanum Tuberosum
• Tetraploidy

PubMed Central ID

• 32449744

Digital Object Identifier (DOI)

• 10.3791/61067

issue

• 159

URL

• http://dx.doi.org/10.3791/61067