Embryo development after vitrification of immature and in vitro-matured equine oocytes. Academic Article uri icon


  • Effects of meiotic stage and cumulus status on development of equine oocytes after vitrification was evaluated. Immature oocytes with corona radiata (IMM); in vitro-matured oocytes with corona radiata (MAT CR+); and in vitro-matured oocytes denuded of cumulus (MAT CR-) were vitrified using the Cryotech method. Warming medium was equilibrated either in 5% CO2 or Air. IMM oocytes underwent in vitro maturation after warming. Recovery, survival, and maturation rates, and cleavage and blastocyst rates after ICSI, were evaluated. Recovery was higher for oocytes warmed in CO2- than Air-equilibrated medium (863 vs. 76.94%, respectively). Maturation for all vitrified-warmed oocyte treatments (376.5 to 45.95.8%) was not different from control (504.1%), except for MAT CR- CO2 (20.34.6%). Cleavage for MAT CR- CO2 and Air groups was similar to control (67.712.1, 71.48.1, and 785.3%, respectively). One blastocyst was produced (MAT CR+CO2), representing the first equine blastocyst reported after vitrification of an in vitro-matured oocyte.

published proceedings

  • Cryobiology

altmetric score

  • 1.25

author list (cited authors)

  • Angel, D., Canesin, H. S., Brom-de-Luna, J. G., Morado, S., Dalvit, G., Gomez, D., ... Velez, I. C.

citation count

  • 8

complete list of authors

  • Angel, Daniel||Canesin, Heloisa Siqueira||Brom-de-Luna, Joao Gatto||Morado, Sergio||Dalvit, Gabriel||Gomez, Diana||Posada, Natalia||Pascottini, Osvaldo Bogado||Urrego, Rodrigo||Hinrichs, Katrin||Velez, Isabel Catalina

publication date

  • January 2020