Cell-specific three-photon-fluorescence brain imaging: neurons, astrocytes, and gliovascular interfaces. Academic Article uri icon


  • We present brain imaging experiments on rat cortical areas, demonstrating that, when combined with a suitable high-brightness, cell-specific genetically encoded fluorescent marker, three-photon-excited fluorescence (3PEF), enables subcellular-resolution, cell-specific 3D brain imaging that is fully compatible and readily integrable with other nonlinear-optical imaging modalities, including two-photon-fluorescence and harmonic-generation microscopy. With laser excitation provided by sub-100-fs, 1.25-m laser pulses, cell-specific 3PEF from astrocytes and their processes detected in parallel with a three-photon-resonance-enhanced third harmonic from blood vessels is shown to enable a high-contrast 3D imaging of gliovascular interfaces.

published proceedings

  • Opt Lett

altmetric score

  • 2.25

author list (cited authors)

  • Lanin, A. A., Pochechuev, M. S., Chebotarev, A. S., Kelmanson, I. V., Bilan, D. S., Kotova, D. A., ... Zheltikov, A. M.

citation count

  • 17

complete list of authors

  • Lanin, AA||Pochechuev, MS||Chebotarev, AS||Kelmanson, IV||Bilan, DS||Kotova, DA||Tarabykin, VS||Ivanov, AA||Fedotov, AB||Belousov, VV||Zheltikov, AM

publication date

  • February 2020