Effects of glucose concentration in semen extender and storage temperature on stallion sperm quality following long-term cooled storage.
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abstract
In Experiment 1, the effects of glucose concentration in extender (0mM, 67mM, 147mM, 270mM; G0, G67, G147, and G270, respectively) and storage temperature of extended semen (5, 10, 15 and 20C) were evaluated after storage for up to 5 days (T0h to T120h). For all time points tested, mean total (TMOT) and progressive (PMOT) sperm motility were lower in G0 than all other treatment groups (P<0.05). Mean curvilinear velocity (VCL) was lower in G0 than other treatment groups at all time points tested except T0h (P<0.05). Mean percentage of plasma membrane/acrosome intact sperm (VAI) was similar among treatments at T0h, T72h, and T120h (P>0.05). Mean TMOT and PMOT, were lower for semen stored at 20C than all lower storage temperatures (P<0.05) at all time points. In Experiment 2, semen was stored at 10C in extender containing no added glucose (G0) or 147mM glucose (G147). Following storage, semen was centrifuged and resuspended in extender containing no added glucose (G0 - G0 or G147 - G0, respectively) or 147mM of glucose (G0 - G147 or G147 - G147, respectively). Mean TMOT, PMOT, and VCL were higher in G147 than G0 at all time periods tested (P<0.05), whereas mean VAI was similar between these treatment groups throughout the experiment (P>0.05). Mean TMOT and PMOT were higher in G0 - G147 than G0 - G0 at T72h and T120h (P<0.05) and mean VCL was higher in G0 - G147 than G0 - G0 for all time periods. Mean TMOT, PMOT, and VCL were higher in G147 - G147 than G147 - G0 at all time points tested (P<0.05), whereas mean VAI was similar between these two treatment groups for each of the time points (P>0.05). In Experiment 3, the minimum concentration of glucose required to maintain sperm quality following long-term cooled storage (T120h) was evaluated (G0, G5, G10, G20, G40, G67, G147 mM). At T120h, mean TMOT was lowest in G0, G5, G10, and G20 (P<0.05), whereas mean PMOT and VCL were lower in G0, G5, G10, and G20 than in G40, G67, and G147 (P<0.05). Mean VAI was higher in G10 than G67, but similar among G10 and other treatment groups (P>0.05). In conclusion, the absence of added glucose in extender reduced the motion characteristics of stallion sperm during long-term storage (5 days), but VAI was not affected. The use of temperatures between 5 and 15C for long-term storage (5 days) best maintained sperm motility and VAI. The threshold concentration of added glucose in extender required to optimize sperm motion characteristics was 40mM.
