LAB/NTAL facilitates fungal/PAMP-induced IL-12 and IFN- production by repressing -catenin activation in dendritic cells. Academic Article uri icon

abstract

  • Fungal pathogens elicit cytokine responses downstream of immunoreceptor tyrosine-based activation motif (ITAM)-coupled or hemiITAM-containing receptors and TLRs. The Linker for Activation of B cells/Non-T cell Activating Linker (LAB/NTAL) encoded by Lat2, is a known regulator of ITAM-coupled receptors and TLR-associated cytokine responses. Here we demonstrate that LAB is involved in anti-fungal immunity. We show that Lat2-/- mice are more susceptible to C. albicans infection than wild type (WT) mice. Dendritic cells (DCs) express LAB and we show that it is basally phosphorylated by the growth factor M-CSF or following engagement of Dectin-2, but not Dectin-1. Our data revealed a unique mechanism whereby LAB controls basal and fungal/pathogen-associated molecular patterns (PAMP)-induced nuclear -catenin levels. This in turn is important for controlling fungal/PAMP-induced cytokine production in DCs. C. albicans- and LPS-induced IL-12 and IL-23 production was blunted in Lat2-/- DCs. Accordingly, Lat2-/- DCs directed reduced Th1 polarization in vitro and Lat2-/- mice displayed reduced Natural Killer (NK) and T cell-mediated IFN- production in vivo/ex vivo. Thus our data define a novel link between LAB and -catenin nuclear accumulation in DCs that facilitates IFN- responses during anti-fungal immunity. In addition, these findings are likely to be relevant to other infectious diseases that require IL-12 family cytokines and an IFN- response for pathogen clearance.

published proceedings

  • PLoS Pathog

author list (cited authors)

  • Orr, S. J., Burg, A. R., Chan, T., Quigley, L., Jones, G. W., Ford, J. W., ... McVicar, D. W.

complete list of authors

  • Orr, Selinda J||Burg, Ashley R||Chan, Tim||Quigley, Laura||Jones, Gareth W||Ford, Jill W||Hodge, Deborah||Razzook, Catherine||Sarhan, Joseph||Jones, Yava L||Whittaker, Gillian C||Boelte, Kimberly C||Lyakh, Lyudmila||Cardone, Marco||O'Connor, Geraldine M||Tan, Cuiyan||Li, Hongchuan||Anderson, Stephen K||Jones, Simon A||Zhang, Weiguo||Taylor, Philip R||Trinchieri, Giorgio||McVicar, Daniel W

editor list (cited editors)

  • Levitz, S. M.

publication date

  • January 1, 2013 11:11 AM