Effects of anticoagulants on stable-isotope values (13 C and 15 N) of shark blood components. Academic Article

abstract

• The effects of anticoagulant EDTA and sodium heparin (SH) on stable carbon 13 C and nitrogen 15 N isotopic values of red blood cells (RBC) and blood plasma in juvenile blacktip reef sharks Carcharhinus melanopterus were analysed. Plasma preserved with anticoagulants was not isotopically distinct from plasma stored in no-additive control tubes but RBC 15 N values exhibited small enrichments when preserved with EDTA and SH. Results suggest EDTA and SH are viable anticoagulants for stable isotopic analyses of blood fractions but further studies are advised to validate results.

authors

• Matich, Philip

published proceedings

• J Fish Biol

altmetric score

• 11.2

author list (cited authors)

• Weideli, O. C., Kiszka, J. J., Matich, P., & Heithaus, M. R.

citation count

• 1

complete list of authors

• Weideli, Ornella C||Kiszka, Jeremy J||Matich, Philip||Heithaus, Michael R

publication date

• December 2019

publisher

• Wiley  Publisher

published in

• Journal of Fish Biology  Journal

keywords

• Animals
• Anticoagulants
• Blood Plasma
• Blood Preservatives
• Carbon Isotopes
• Carcharhinus Melanopterus
• Edetic Acid
• Erythrocytes
• Heparin
• Nitrogen Isotopes
• Plasma
• Red Blood Cells (rbc)
• Sharks
• Specimen Handling
• Δ13c
• δ15n

PubMed Central ID

• 31621068

Digital Object Identifier (DOI)

• 10.1111/jfb.14164

start page

• 1535

end page

• 1539

volume

• 95

issue

• 6

URL

• http://dx.doi.org/10.1111/jfb.14164