Critical Comparison of the Superoxide Dismutase-like Activity of Carbon Antioxidant Nanozymes by Direct Superoxide Consumption Kinetic Measurements. Academic Article uri icon


  • The superoxide dismutase-like activity of poly(ethylene glycolated) hydrophilic carbon clusters (PEG-HCCs), anthracite and bituminous graphene quantum dots (PEG-aGQDs and PEG-bGQDs, respectively), and two fullerene carbon nanozymes, tris malonyl-C60 fullerene (C3) and polyhydroxylated-C60 fullerene (C60-OHn), were compared using direct optical stopped-flow kinetic measurements, together with three native superoxide dismutases (SODs), CuZnSOD, MnSOD, and FeSOD, at both pH 12.7 and 8.5. Computer modeling including both SOD catalytic steps and superoxide self-dismutation enabled the best choice of catalyst concentration with minimal contribution to the observed kinetic change from the substrate self-dismutation. Biexponential fitting to the kinetic data ranks the rate constant (M-1 s-1) in the order of PEG-HCCs > CuZnSOD MnSOD PEG-aGQDs PEG-bGQDs > FeSOD C3 > C60-OHn at pH 12.7 and MnSOD > CuZnSOD PEG-HCCs > FeSOD > PEG-aGQDs PEG-bGQDs C3 C60-OHn at pH 8.5. Nonlinear regression of the kinetic model above yielded the same ranking as the biexponential fit, but provided better mechanistic insight. The data obtained by freeze-quench EPR direct assay at pH 12.7 also yield the same ranking as stopped-flow data. This is a necessary assessment of a panel of proclaimed carbon nano SOD mimetics using the same two direct methods, revealing a dramatic, 3-4 orders of magnitude difference in SOD activity between PEG-HCCs/PEG-GQDs from soluble fullerenes.

published proceedings

  • ACS Nano

altmetric score

  • 1.25

author list (cited authors)

  • Wu, G., Berka, V., Derry, P. J., Mendoza, K., Kakadiaris, E., Roy, T., ... Tsai, A.

citation count

  • 25

complete list of authors

  • Wu, Gang||Berka, Vladimir||Derry, Paul J||Mendoza, Kimberly||Kakadiaris, Eugenia||Roy, Trenton||Kent, Thomas A||Tour, James M||Tsai, Ah-Lim

publication date

  • October 2019